Product Name :
PCNA Recombinant Rabbit Monoclonal Antibody [SY12-07]
Predicted band size :
29 kDa
Observed band size :
34 kDa
Synonyms:
ATLD2 antibody cb16 antibody Cyclin antibody DNA polymerase delta auxiliary protein antibody etID36690.10 antibody fa28e03 antibody fb36g03 antibody HGCN8729 antibody MGC8367 antibody Mutagen-sensitive 209 protein antibody 展开
Function :
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within Human PCNA aa 88-137 / 261.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Coupling :
unconjugated
Clone number:
SY12-07
RRID:
AB_2941955
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.1,3-Diisopropylimidazolium chloride custom synthesis Aliquot store at -20℃ or -80℃.Ni(COD)2 site Avoid repeated freeze / thaw cycles.PMID:33530921
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 29 kDa
Positive control :
HEK-293 cell lysate, HeLa cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, HeLa, human tonsil tissue, human spleen tissue, human breast carcinoma tissue, mouse colon tissue, mouse spleen tissue, mouse stomach tissue.